Medium data

Search for medium no. = [740].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

740   MVL MEDIUM

Mineral solution 1 (see below)  75.0     ml
Mineral solution 2 (see below)  75.0     ml
0.1% Resazurin solution  1.0     ml
Trypticase peptone (BD-BBL)  10.0     g
Yeast extract (BD-Difco)  5.0     g
Beef extract  2.0     g
Agar  15.0     g
Glucose  2.0     g
0.07% Hemin solution  10.0     ml
8.0% Na2CO3 solution  50.0     ml
3.0% L-Cysteine·HCl·H2O solution  10.0     ml
Distilled water  1.0     L
Mineral solution 1:
0.6% KH2PO4 solution.
Mineral solution 2:
K2HPO4  0.6     g
(NH4)2SO4  1.2     g
NaCl  1.2     g
MgSO4·7H2O  0.12    g
CaCl2·2H2O  0.12    g
Distilled water  100.0     ml