Medium data
Search for medium no. = [730].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
730 RYE-BRAN MRS
| Lactobacilli MRS broth (BD-Difco) | 55.0 | g |
| Rye-bran extract (see below) | 900.0 | ml |
| Distilled water | 100.0 | ml |
Rye-bran extract:
| Rye-bran | 40.0 | g |
| Malted wheat meal | 2.0 | g |
| Trypsin (Sigma-Aldrich) | 0.8 | g |
| Distilled water | 1.0 | L |
Mix components and incubate at 50C for 24 hr. Clarify the resulting turbid liquid by centrifugation (500 x g, 15 min) followed by paper-filtration.
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