Medium data

Search for medium no. = [724].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

724   RAVOT MODIFIED MEDIUM FOR MARINITOGA LITORALIS

NaCl  26.0     g
MgCl2·6H2O  0.5     g
KCl  0.5     g
NH4Cl  0.3     g
KH2PO4  0.1     g
CaCl2·2H2O  0.1     g
Sodium acetate·3H2O  0.83    g
Yeast extract  2.0     g
Trypticase peptone (BD-BBL)  2.0     g
Maltose  2.0     g
PIPES  3.3     g
Sulfur (powder)  5.0     g
Resazurin  1.0     mg
Distilled water  1.0     L
Mix components except sulfur, adjust pH to 6.0 and autoclave under a N2 atmosphere. After cooling, add per liter the following solutions (autoclaved and stored anaerobically):
7% KH2PO4solution  4.3     ml
7% K2HPO4solution  4.3     ml
Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels containing sulfur under a N2 atmosphere, and seal with butyl rubber stoppers. Then, add the following solution (autoclaved and stored under a N2 atmosphere) to the medium:
3% Na2S·9H2O solution  10.0     ml