Medium data
Search for medium no. = [723].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
723 ANAEROBIC BRAIN HEART INFUSION BROTH
| Brain heart infusion broth (BD-Difco) | 37.0 | g |
| Distilled water | 1.0 | L |
Prepare the medium anaerobically under a N2 atmosphere. Prior to inoculation, add per liter the following solutions (autoclaved and stored under a N2 atmosphere) aseptically and anaerobically:
| 5% L-Cysteine·HCl·H2O | 5.0 | ml |
| 5% Na2S·9H2O | 5.0 | ml |
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