Medium data
Search for medium no. = [713].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
713 THERMOVORAX SUBTERRANEUS MEDIUM
Solution A:
| KH2PO4 | 0.42 | g |
| Na2HPO4 | 0.43 | g |
| Glucose | 5.0 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 800.0 | ml |
Mix components thoroughly. Distribute into culture vessels under a N2 gas atmosphere (e.g., 45 ml in 100 ml serum bottle), seal with butyl rubber stoppers and autoclave.
Solution B:
| NH4Cl | 0.3 | g |
| NaCl | 0.3 | g |
| CaCl2·2H2O | 0.11 | g |
| MgCl2·6H2O | 0.1 | g |
| Trace mineral solution (see below) | 1.0 | ml |
| Vitamin solution (see below) | 1.0 | ml |
| Se-W solution (see below) | 1.0 | ml |
| Distilled water | 47.0 | ml |
Mix components thoroughly. Filter-sterilize and store under a N2 gas atmosphere.
Solution C:
| L-Cysteine·HCl·H2O | 0.5 | g |
| Yeast extract (BD-Difco) | 0.2 | g |
| NaHCO3 | 0.4 | g |
| Distilled water | 49.0 | ml |
Mix components thoroughly. Filter-sterilize and store under a N2 gas atmosphere. Then add the following solution (filter-sterilized) aseptically and anaerobically:
To complete medium, add 2.5 ml Solution B and 2.5 ml Solution C to the culture vessels containing 45.0 ml of the medium (Solution A).
Trace mineral solution:
| 25% HCl (7.7 M) | 10.0 | ml |
| FeCl2·4H2O | 2.0 | g |
| H3BO3 | 0.05 | g |
| ZnCl2 | 0.05 | g |
| CuCl2·2H2O | 0.04 | g |
| MnCl2·4H2O | 0.04 | g |
| (NH4)6Mo7O7·4H2O | 0.05 | g |
| AlCl3 | 0.05 | g |
| CoCl2·6H2O | 0.05 | g |
| NiCl2·6H2O | 0.08 | g |
| EDTA | 0.5 | g |
| Distilled water | 987.0 | ml |
Dissolve FeCl2·4H2O in HCl solution at first. Then add 300 ml distilled water and dissolve the remaining components. Fill up to 1.0 L.
Vitamin solution:
| Biotin | 2.0 | mg |
| Folic acid | 2.0 | mg |
| Pyridoxine·HCl | 10.0 | mg |
| Thiamine·HCl | 5.0 | mg |
| Riboflavin | 5.0 | mg |
| Nicotinic acid | 10.0 | mg |
| DL-Calcium pantothenate | 5.0 | mg |
| Vitamin B12 | 5.0 | mg |
| p-Aminobenzoic acid | 5.0 | mg |
| Lipoic acid | 5.0 | mg |
| Distilled water | 100.0 | ml |
Se-W solution:
| 10 mM NaOH solution | 10.0 | ml |
| Na2WO4·2H2O | 26.5 | mg |
| Na2SeO3·5H2O | 33.0 | mg |
| Distilled water | 990.0 | ml |
Dissolve Na2WO4·2H2O and Na2SeO3·5H2O in 10 mM NaOH solution at first. Then dilute with distilled water.
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