Medium data
Search for medium no. = [705].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
705 SELENOMONAS WG MEDIUM
| KH2PO4 | 0.5 | g |
| K2HPO4 | 0.5 | g |
| (NH4)2SO4 | 1.0 | g |
| NaCl | 1.0 | g |
| MgSO4·7H2O | 0.1 | g |
| CaCl2·2H2O | 0.1 | g |
| Tryptone (BD-Difco) | 5.0 | g |
| Yeast extract | 2.0 | g |
| Glucose | 2.0 | g |
| VFA solution (see below) | 10.0 | ml |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix components and adjust pH to 7.0. Bring to a boil for several seconds and cool down to room temperature under a stream of N2-CO2 (4:1, v/v). Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. Prior to use, add per liter the following sterile solutions (autoclaved):
| 5% L-Cysteine·HCl·H2O | 5.0 | ml |
| 5% Na2S·9H2O | 5.0 | ml |
VFA solution:
| Butyric acid | 1.0 | ml |
| iso-Valeric acid | 1.0 | ml |
| n-Valeric acid | 1.0 | ml |
| Distilled water | 100.0 | ml |
Adjust pH to 6.7 - 6.8, and store in a tightly stoppered bottle at 4C.
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