Medium data
Search for medium no. = [704].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
704 ANOXYBACILLUS TUNISIENSE MEDIUM
| Base solution A (see below) | 100.0 | ml |
| Base solution B (see below) | 20.0 | ml |
| Tryptone (BD-Difco) | 2.5 | g |
| Yeast extract (BD-Difco) | 2.5 | g |
| Distilled water | 880.0 | ml |
Adjust pH to 8.0.
Base solution A:
| Nitrilotriacetic acid | 1.32 | g |
| MgCl2·6H2O | 0.2 | g |
| CaSO4·2H2O | 0.4 | g |
| Trace elements (see below) | 5.0 | ml |
| Fe citrate solution (see below) | 5.0 | ml |
| Distilled water | 990.0 | ml |
Adjust pH to 7.2.
Base solution B:
| KH2PO4 | 5.44 | g |
| Na2HPO4·2H2O | 21.4 | g |
| Distilled water | 1.0 | L |
Trace elements:
| Nitrilotriacetic acid | 12.8 | g |
| FeCl2·4H2O | 1.0 | g |
| MnCl2·4H2O | 0.5 | g |
| CoCl2·6H2O | 0.3 | g |
| ZnCl2 | 0.2 | g |
| CuCl2·2H2O | 0.05 | g |
| Na2MoO4·2H2O | 0.05 | g |
| H3BO3 | 0.02 | g |
| NiCl2·6H2O | 0.02 | g |
| Distilled water | 1.0 | L |
Adjust pH to 6.0 - 6.5.
Fe citrate solution:
| Sodium citrate·2H2O | 2.94 | g |
| FeCl3·6H2O | 2.7 | g |
| Distilled water | 1.0 | L |
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