Medium data
Search for medium no. = [691].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
691 AEROBIC SULFOLOBALES MEDIUM
| Salt base solution (see below) | 1.0 | L |
| Yeast extract (BD-Difco) | 0.5 | g |
| Sulfur (powder) | 1.0 | g |
Autoclave Salt base solution and yeast extract (as a 10% solution) separately and combine after cooling. Steam sulfur for 3 hr on each of 3 successive days.
Salt base solution:
| (NH4)2SO4 | 1.3 | g |
| KH2PO4 | 0.28 | g |
| MgSO4·7H2O | 0.25 | g |
| CaCl2·2H2O | 0.07 | g |
| MnCl2·4H2O | 1.8 | mg |
| Na2B4O7·10H2O | 4.5 | mg |
| ZnSO4·7H2O | 0.22 | mg |
| CuCl2·2H2O | 0.05 | mg |
| Na2MoO4·2H2O | 0.03 | mg |
| VOSO4·xH2O | 0.03 | mg |
| CoSO4·7H2O | 0.01 | mg |
| Distilled water | 1.0 | L |
Adjust pH to 2.5 with 10 N H2SO4.
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