Medium data

Search for medium no. = [691].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

691   AEROBIC SULFOLOBALES MEDIUM

Salt base solution (see below)  1.0     L
Yeast extract (BD-Difco)  0.5     g
Sulfur (powder)  1.0     g
Autoclave Salt base solution and yeast extract (as a 10% solution) separately and combine after cooling. Steam sulfur for 3 hr on each of 3 successive days.
Salt base solution:
(NH4)2SO4  1.3     g
KH2PO4  0.28    g
MgSO4·7H2O  0.25    g
CaCl2·2H2O  0.07    g
MnCl2·4H2O  1.8     mg
Na2B4O7·10H2O  4.5     mg
ZnSO4·7H2O  0.22    mg
CuCl2·2H2O  0.05    mg
Na2MoO4·2H2O  0.03    mg
VOSO4·xH2O  0.03    mg
CoSO4·7H2O  0.01    mg
Distilled water  1.0     L
Adjust pH to 2.5 with 10 N H2SO4.



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