Medium data

Search for medium no. = [685].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

685   ALICYCLOBACILLUS FERROOXYDANS MEDIUM

Solution A:
MgSO4·7H2O  0.5     g
(NH4)2SO4  0.4     g
K2HPO4  0.2     g
K2S4O6  0.15    g
KCl  0.1     g
Yeast extract  0.2     g
Distilled water  1.0     L
Adjust pH to 2.5 with H2SO4 and autoclave.

Solution B:
FeSO4·7H2O  20.0     g
Dissolve in final 100 ml of 0.2 N H2SO4 and filter-sterilize.

To complete the medium, add 70 ml Solution B to 1.0 L Solution A. For solid medium, prepare double-strength Solution A and autoclave. Separately autoclave an equal amount of 1.4% Gerlite (gellan gum) solution. After autoclaving, keep the two solutions at 70C and mix thoroughly. Then, add filter-sterilized Solution B (70 ml/L) and pour into petri dishes.



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