Medium data
Search for medium no. = [685].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
685 ALICYCLOBACILLUS FERROOXYDANS MEDIUM
Solution A:
MgSO4·7H2O | 0.5 | g |
(NH4)2SO4 | 0.4 | g |
K2HPO4 | 0.2 | g |
K2S4O6 | 0.15 | g |
KCl | 0.1 | g |
Yeast extract | 0.2 | g |
Distilled water | 1.0 | L |
Adjust pH to 2.5 with H2SO4 and autoclave.
Solution B:
Dissolve in final 100 ml of 0.2 N H2SO4 and filter-sterilize.
To complete the medium, add 70 ml Solution B to 1.0 L Solution A. For solid medium, prepare double-strength Solution A and autoclave. Separately autoclave an equal amount of 1.4% Gerlite (gellan gum) solution. After autoclaving, keep the two solutions at 70C and mix thoroughly. Then, add filter-sterilized Solution B (70 ml/L) and pour into petri dishes.
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