Medium data
Search for medium no. = [675].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
675 RUMINOCOCCUS ALBUS MEDIUM
| Tryptone (BD-Difco) | 5.0 | g |
| Yeast extract (BD-Difco) | 2.0 | g |
| Glucose | 3.0 | g |
| Cellobiose | 2.0 | g |
| Mineral solution 1 (see below) | 40.0 | ml |
| Mineral solution 2 (see below) | 40.0 | ml |
| Resazurin | 1.0 | mg |
| Distilled water | 920.0 | ml |
Boil and cool under CO2. Under a gentle stream of CO2, add 4.0 g Na2CO3, 1.0 ml fatty acid mixture (see below) and 500 mg L-cysteine·HCl·H2O.
Adjust pH to 7.0, distribute into appropriate vessels under N2 and autoclave.
Mineral solution 1:
| K2HPO4 | 0.6 | g |
| Distilled water | 100.0 | ml |
Mineral solution 2:
| KH2PO4 | 0.6 | g |
| (NH4)2SO4 | 2.0 | g |
| NaCl | 1.2 | g |
| MgSO4·7H2O | 0.25 | g |
| CaCl2·2H2O | 0.16 | g |
| Distilled water | 100.0 | ml |
Fatty acid mixture:
| Isobutyric acid | 10.0 | ml |
| Isovaleric acid | 10.0 | ml |
| 2-Methylbutyric acid | 10.0 | ml |
| Distilled water | 70.0 | ml |
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