Medium data

Search for medium no. = [675].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

675   RUMINOCOCCUS ALBUS MEDIUM

Tryptone (BD-Difco)  5.0     g
Yeast extract (BD-Difco)  2.0     g
Glucose  3.0     g
Cellobiose  2.0     g
Mineral solution 1 (see below)  40.0     ml
Mineral solution 2 (see below)  40.0     ml
Resazurin  1.0     mg
Distilled water  920.0     ml
Boil and cool under CO2. Under a gentle stream of CO2, add 4.0 g Na2CO3, 1.0 ml fatty acid mixture (see below) and 500 mg L-cysteine·HCl·H2O.
Adjust pH to 7.0, distribute into appropriate vessels under N2 and autoclave.
Mineral solution 1:
K2HPO4  0.6     g
Distilled water  100.0     ml
Mineral solution 2:
KH2PO4  0.6     g
(NH4)2SO4  2.0     g
NaCl  1.2     g
MgSO4·7H2O  0.25    g
CaCl2·2H2O  0.16    g
Distilled water  100.0     ml
Fatty acid mixture:
Isobutyric acid  10.0     ml
Isovaleric acid  10.0     ml
2-Methylbutyric acid  10.0     ml
Distilled water  70.0     ml



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