Medium data

Search for medium no. = [674].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

674   PYG MEDIUM (F)

S I solution (see below)  75.0     ml
S II solution (see below)  75.0     ml
Trypticase peptone  10.0     g
Yeast extract  5.0     g
Glucose  10.0     g
0.1% (w/v) Sodium resazurin solution  1.0     ml
8% (w/v) Na2CO3 solution  2.5     ml
L-Cysteine·HCl·H2O  0.3     g
Distilled water  850.0     ml
Adjust pH to 7.3.
S I solution:
K2HPO4  0.6     g
Distilled water  100.0     ml
S II solution:
KH2PO4  0.6     g
(NH4)2SO4  1.2     g
NaCl  1.2     g
MgSO4·7H2O  0.12    g
CaCl2·2H2O  0.12    g
Distilled water  100.0     ml