Medium data
Search for medium no. = [674].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
674 PYG MEDIUM (F)
| S I solution (see below) | 75.0 | ml |
| S II solution (see below) | 75.0 | ml |
| Trypticase peptone | 10.0 | g |
| Yeast extract | 5.0 | g |
| Glucose | 10.0 | g |
| 0.1% (w/v) Sodium resazurin solution | 1.0 | ml |
| 8% (w/v) Na2CO3 solution | 2.5 | ml |
| L-Cysteine·HCl·H2O | 0.3 | g |
| Distilled water | 850.0 | ml |
Adjust pH to 7.3.
S I solution:
| K2HPO4 | 0.6 | g |
| Distilled water | 100.0 | ml |
S II solution:
| KH2PO4 | 0.6 | g |
| (NH4)2SO4 | 1.2 | g |
| NaCl | 1.2 | g |
| MgSO4·7H2O | 0.12 | g |
| CaCl2·2H2O | 0.12 | g |
| Distilled water | 100.0 | ml |
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