Medium data
Search for medium no. = [662].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
662 MBG-20
| Peptone | 16.0 | g |
| Yeast extract | 4.0 | g |
| Glucose | 5.0 | g |
| Sea salts (Sigma) | 40.0 | g |
| Distilled water | 1.0 | L |
Adjust pH to 7.0.
Dissolve Sea salts in distilled water and sterilize by filtration. Separately, autoclave peptone, yeast extract and glucose solutions and add to the sterile Sea salts solution. Make the liquid medium anaerobic by repeated cycles (three times) of degassing and charging with argon.
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