Medium data
Search for medium no. = [659].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
659 PELAGICOCCUS AGAR
| Marine agar 2216 (BD-Difco) | 55.1 | g |
| R2A agar (BD-Difco) | 9.1 | g |
| Artificial seawater (see below) | 750.0 | ml |
| Distilled water | 250.0 | ml |
Artificial seawater:
| NaCl | 24.0 | g |
| MgSO4·7H2O | 7.0 | g |
| MgCl2·6H2O | 5.3 | g |
| KCl | 0.7 | g |
| CaCl2·2H2O | 0.1 | g |
| Distilled water | 1.0 | L |
Adjust pH to 7.5.
Comment: For preparation of the liquid medium, dissolve 37.4 g/L Marine Broth 2216 (BD-Difco) and 1.6 g/L R2A broth (Daigo) in the artificial sea water.
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