Medium data

Search for medium no. = [654].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

654   POSTGATE J. R'S MEDIUM C

KH2PO4  0.5     g
NH4Cl  1.0     g
Na2SO4  4.5     g
CaCl2·2H2O  0.06    g
MgSO4·7H2O  0.06    g
Yeast extract  1.0     g
FeSO4·7H2O  4.0     mg
Sodium citrate  0.3     g
Distilled water  900.0     ml
Mix components thoroughly and autoclave. Then add the following solution:
6.0% Sodium lactate solution (filter-sterilized)  100.0     ml
Cultivate under a N2 atmosphere.