Medium data

Search for medium no. = [653].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

653   SYMBIOBACTERIUM MEDIUM

Tryptone (BD-Difco)  10.0     g
Yeast extract (BD-Difco)  5.0     g
NaCl  5.0     g
NaNO3  1.7     g
Distilled water  900.0     ml
Mix components and adjust pH to 8.0. Distribute the medium into culture vessels and replace the gas phase with a N2-CO2-H2 (80:10:10, v/v) gas mixture. Seal with butyl rubber stoppers and autoclave. After cooling, aseptically add the following solution (filter-sterilized) to the medium:
0.4 M NH4HCO3 solution  100.0     ml
For preparation of solid medium, add 10.0 g/L gellan gum.