Medium data

Search for medium no. = [639].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


Solution A:
(NH4)2SO4  0.9     g
NaCl  0.9     g
MgSO4·7H2O  0.9     g
CaCl2·2H2O  0.9     g
Cellobiose  2.5     g
Rumen fluid, clarified (see Medium No. 266)  200.0     ml
Distilled water  600.0     ml
Solution B:
KH2PO4  0.45    g
K2HPO4  0.45    g
Distilled water  100.0     ml
Solution C:
NaHCO3  3.5     g
Distilled water  100.0     ml
Autoclave Solutions A and B separately and filter-sterilize Solution C. Combine the three solutions, distribute into culture vessels under a stream of N2-CO2 (80:20, v/v) and seal with butyl rubber stoppers. Prior to inoculation, reduce the medium with 0.05% L-cysteine·HCl·H2O (autoclaved as a 5% solution under a N2 atmosphere).

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