Medium data

Search for medium no. = [626].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

626   FERVIDOBACTERIUM TIANCHISUM MEDIUM

(NH4)2SO4  1.3     g
KH2PO4  0.28    g
MgSO4·7H2O  0.25    g
CaCl2·2H2O  0.07    g
MnCl2·4H2O  1.8     mg
Na2B4O7·10H2O  4.5     mg
ZnSO4·7H2O  0.22    mg
CuSO4·5H2O  0.05    mg
Na2MoO4·2H2O  0.03    mg
CoCl2·6H2O  0.01    mg
Glucose  5.0     g
Tryptone (BD-Difco)  4.0     g
Na2S·9H2O  0.5     g
Sulfur (powder)  5.0     g
Distilled water  1.0     L
Mix ingredients except glucose, sulfur and Na2S·9H2O, and autoclave under a N2 atmosphere. Separately autoclave glucose as a 20% solution and Na2S·9H2O as a 5% solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Aseptically and anaerobically add glucose and sulfur to the medium. Prior to inoculation, add sterile Na2S·9H2O solution and adjust pH to 7.5 with sterile 1 N H2SO4.



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