Medium data
Search for medium no. = [623].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
623
Solution A:
Yeast extract (BD-Difco) | 10.0 | g |
Tryptone (BD-Difco) | 2.5 | g |
Agar | 20.0 | g |
Distilled water | 500.0 | ml |
Adjust pH to 7.4.
Solution B:
NaCl | 120.0 | g |
MgSO4·7H2O | 10.0 | g |
KCl | 5.0 | g |
CaCl2·2H2O | 0.2 | g |
Distilled water | 500.0 | ml |
Separately autoclave Solutions A and B and mix them aseptically. Adjust pH to 7.4, if necessary.
Copyright © 2025 Microbe Division (JCM) - All Rights Reserved