Medium data

Search for medium no. = [618].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

618 CYS MEDIUM

N-Z-Case (Wako)	`3.0`	g
Yeast extract (BD-Difco)	`2.0`	g
Soluble starch (BD-Difco)	`1.0`	g
NaCl	`20.0`	g
MgCl₂·6H₂O	`0.125`	g
CaCl₂·2H₂O	`0.025`	g
FeSO₄·7H₂O	`0.01`	g
Distilled water	`900.0`	ml

Add components to distilled water and mix thoroughly. Then, add the following solutions in order listed:

Na₂MoO₄·2H₂O (12 g/L)	`100.0`	µl
VOSO₄·xH₂O (1 g/L)	`100.0`	µl
MnCl₂·4H₂O (5.0 g/L in 0.01 N H₂SO₄)	`100.0`	µl
ZnSO₄·7H₂O (0.6 g/L in 0.01 N H₂SO₄)	`100.0`	µl
CuSO₄·5H₂O (0.15 g/L in 0.01 N H₂SO₄)	`100.0`	µl
CoCl₂·6H₂O (8.0 g/L in 0.01 N H₂SO₄)	`100.0`	µl
NiCl₂·6H₂O (0.2 g/L in 0.01 N H₂SO₄)	`100.0`	µl

Adjust pH to 7.5, bring volume to 1.0 L with distilled water and autoclave.
Comment: Inoculate into the medium prewarmed to the optimal growth temperature.