Medium data
Search for medium no. = [616].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
616 DESULFOMICROBIUM MEDIUM
| KH2PO4 | 0.25 | g |
| NH4Cl | 0.5 | g |
| CaCl2·2H2O | 0.05 | g |
| MgSO4·7H2O | 0.5 | g |
| KCl | 0.5 | g |
| Na2SO4 | 1.42 | g |
| Sodium lactate | 1.12 | g |
| Trace element solution SL12B (see below) | 1.0 | ml |
| Distilled water | 1.0 | L |
Mix ingredients and autoclave. Cool under a N2-CO2 gas mixture (4:1, v/v) and add the following solutions from sterile stocks:
| 8% NaHCO3 solution | 30.0 | ml |
| 5% Na2S·9H2O solution | 1.0 | ml |
| Vitamin solution (see below) | 1.0 | ml |
Distribute the medium into culture vessels under the same gas mixture and seal with butyl rubber stoppers. Adjust pH to 7.0 - 7.2, if necessary.
Trace element solution SL12B:
| EDTA·2Na | 3.0 | g |
| FeSO4·7H2O | 1.10 | g |
| CoCl2·6H2O | 190.0 | mg |
| MnCl4·4H2O | 50.0 | mg |
| ZnCl2 | 42.0 | mg |
| NiCl2·6H2O | 24.0 | mg |
| Na2MoO4·2H2O | 18.0 | mg |
| H3BO3 | 300.0 | mg |
| CuCl2·2H2O | 2.0 | mg |
| Distilled water | 1.0 | L |
Vitamin solution:
| Vitamin B12 | 100.0 | mg |
| p-Aminobenzoic acid | 80.0 | mg |
| Biotin | 20.0 | mg |
| Nicotinic acid | 200.0 | mg |
| DL-Calcium pantothenate | 100.0 | mg |
| Pyridoxine·HCl | 300.0 | mg |
| Thiamine·HCl | 200.0 | mg |
| Distilled water | 1.0 | L |
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