Medium data
Search for medium no. = [611].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
611
| Casamino acids (BD-Difco) | 5.0 | g |
| Yeast extract (BD-Difco) | 5.0 | g |
| Sodium glutamate | 1.0 | g |
| Trisodium citrate | 3.0 | g |
| KCl | 2.0 | g |
| MgSO4·7H2O | 0.2 | g |
| FeCl2·4H2O | 36.0 | mg |
| NaCl | 100.0 | g |
| Bacto agar (BD-Difco) | 20.0 | g |
| Distilled water | 900.0 | ml |
Adjust pH to 7.3.
After autoclaving, aseptically add 100 ml of 10% Na2CO3 solution to the medium. Check final pH to be about 9.5.
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