Medium data
Search for medium no. = [599].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
599 MODERATE HALOPHILE MEDIUM
| Yeast extract | 10.0 | g |
| Proteose peptone No. 3 (BD-Difco) | 5.0 | g |
| Glucose | 1.0 | g |
| NaCl | 81.0 | g |
| MgSO4·7H2O | 9.6 | g |
| MgCl2·6H2O | 7.0 | g |
| CaCl2·2H2O | 0.36 | g |
| KCl | 2.0 | g |
| NaHCO3 | 0.06 | g |
| NaBr | 0.026 | g |
Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.0 - 7.2. For preparation of solid medium, add 20.0 g/L agar.
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