Medium data

Search for medium no. = [599].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

599   MODERATE HALOPHILE MEDIUM

Yeast extract  10.0     g
Proteose peptone No. 3 (BD-Difco)  5.0     g
Glucose  1.0     g
NaCl  81.0     g
MgSO4·7H2O  9.6     g
MgCl2·6H2O  7.0     g
CaCl2·2H2O  0.36    g
KCl  2.0     g
NaHCO3  0.06    g
NaBr  0.026   g
Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.0 - 7.2. For preparation of solid medium, add 20.0 g/L agar.