Medium data
Search for medium no. = [595].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
595 CLOSTRIDIUM THERMOCELLUM MEDIUM
| (NH4)2SO4 | 1.3 | g |
| MgCl2·6H2O | 2.6 | g |
| KH2PO4 | 1.43 | g |
| K2HPO4·3H2O | 7.2 | g |
| CaCl2·2H2O | 0.13 | g |
| Sodium β-glycerophosphate | 6.0 | g |
| FeSO4·7H2O | 1.1 | mg |
| Glutathione | 0.25 | g |
| Yeast extract | 4.5 | g |
| Resazurin | 1.0 | mg |
| Cellulose (Avicel or MN 300) | 10.0 | g |
| Distilled water | 1.0 | L |
Adjust pH to 7.0 - 7.2.
Gas atmosphere: 95% N2 + 5% CO2.
Comment: Cellulose can be replaced with 5.0 g/L cellobiose. In this case, cellobiose is filter-sterilized separately as 10% (w/v) solution in distilled water.
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