Medium data

Search for medium no. = [595].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

595   CLOSTRIDIUM THERMOCELLUM MEDIUM

(NH4)2SO4  1.3     g
MgCl2·6H2O  2.6     g
KH2PO4  1.43    g
K2HPO4·3H2O  7.2     g
CaCl2·2H2O  0.13    g
Sodium β-glycerophosphate  6.0     g
FeSO4·7H2O  1.1     mg
Glutathione  0.25    g
Yeast extract  4.5     g
Resazurin  1.0     mg
Cellulose (Avicel or MN 300)  10.0     g
Distilled water  1.0     L
Adjust pH to 7.0 - 7.2.
Gas atmosphere: 95% N2 + 5% CO2.
Comment: Cellulose can be replaced with 5.0 g/L cellobiose. In this case, cellobiose is filter-sterilized separately as 10% (w/v) solution in distilled water.