Medium data

Search for medium no. = [591].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

591 HALF STRENGTH TRYPTICASE SOY BROTH AGAR (pH 4.0)

Solution A:

Trypticase soy broth (BD-BBL)	`15.0`	g
Distilled water	`500.0`	ml

Adjust pH to 4.0.
Solution B:

Agar	`15.0`	g
Distilled water	`500.0`	ml

After autoclaving Solutions A and B separately and cooling to 50C, aseptically mix both solutions and dispense into sterile test tubes or petri dishes.