Medium data

Search for medium no. = [589].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

589   PY MEDIUM (C)

Peptone  10.0     g
Yeast extract  10.0     g
Salts solution (see below)  40.0     ml
Distilled water  1.0     L
Mix components thoroughly and autoclave under a N2 atmosphere. Aseptically and anaerobically add the following solutions (autoclaved under a N2 atmosphere) to the medium:
20% Na2CO3 solution  10.0     ml
5% Na2S·9H2O  10.0     ml
Salts solution:
CaCl2·2H2O  0.2     g
MgSO4·7H2O  0.2     g
KH2PO4  1.0     g
K2HPO4  1.0     g
NaHCO3  10.0     g
NaCl  2.0     g
Distilled water  1.0     L



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