Medium data
Search for medium no. = [580].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
580 RHODOBACTER SPHAEROIDES MEDIUM
| KH2PO4 | 0.5 | g |
| MgCl2·6H2O | 0.2 | g |
| NaCl | 0.4 | g |
| NH4Cl | 1.2 | g |
| CaCl2·2H2O | 0.05 | g |
| Sorbitol | 3.0 | g |
| Sodium pyruvate | 3.0 | g |
| Yeast extract | 0.4 | g |
| Distilled water | 1.0 | L |
Adjust pH to 7.0.
Add the following component after autoclaving:
| Trace element solution SL7 (see below) | 1.0 | ml |
| Vitamin B12 solution (20 µg/L) | 1.0 | ml |
Trace element solution SL7:
| HCl (25%, v/v) | 1.0 | ml |
| ZnCl2 | 70.0 | mg |
| MnCl2·4H2O | 100.0 | mg |
| H3BO3 | 60.0 | mg |
| CoCl2·6H2O | 200.0 | mg |
| CuCl2·2H2O | 20.0 | mg |
| NiCl2·6H2O | 20.0 | mg |
| Na2MoO4·2H2O | 40.0 | mg |
| Distilled water | 1.0 | L |
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