Medium data

Search for medium no. = [580].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

580   RHODOBACTER SPHAEROIDES MEDIUM

KH2PO4  0.5     g
MgCl2·6H2O  0.2     g
NaCl  0.4     g
NH4Cl  1.2     g
CaCl2·2H2O  0.05    g
Sorbitol  3.0     g
Sodium pyruvate  3.0     g
Yeast extract  0.4     g
Distilled water  1.0     L
Adjust pH to 7.0.
Add the following component after autoclaving:
Trace element solution SL7 (see below)  1.0     ml
Vitamin B12 solution (20 µg/L)  1.0     ml
Trace element solution SL7:
HCl (25%, v/v)  1.0     ml
ZnCl2  70.0     mg
MnCl2·4H2O  100.0     mg
H3BO3  60.0     mg
CoCl2·6H2O  200.0     mg
CuCl2·2H2O  20.0     mg
NiCl2·6H2O  20.0     mg
Na2MoO4·2H2O  40.0     mg
Distilled water  1.0     L