Medium data
Search for medium no. = [58].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
58 INORGANIC SALTS-STARCH AGAR (ISP-4)
Soluble starch | 10.0 | g |
K2HPO4 | 1.0 | g |
MgSO4·7H2O | 1.0 | g |
NaCl | 1.0 | g |
(NH4)2SO4 | 2.0 | g |
CaCO3 | 2.0 | g |
Trace salts solution (see below) | 1.0 | ml |
Agar | 20.0 | g |
Distilled water | 1.0 | L |
Unadjusted pH will be 7.0-7.4.
Trace salts solution:
FeSO4·7H2O | 0.1 | g |
MnCl2·4H2O | 0.1 | g |
ZnSO4·7H2O | 0.1 | g |
Distilled water | 100.0 | ml |
Comment: The premixed powder is available from Becton Dickinson & Co. as ISP Medium 4.
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