Medium data

Search for medium no. = [568].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

568   ROSEOSPIRA MEDIUM

KH2PO4  0.5     g
MgSO4·7H2O  1.5     g
NaCl  20.0     g
NH4Cl  0.6     g
CaCl2·2H2O  0.15    g
Sodium pyruvate  3.0     g
Yeast extract  0.4     g
Ferric citrate (0.1%, w/v)  5.0     ml
Micronutrient solution (SL7) (see below)  1.0     ml
Distilled water  1.0     L
Adjust pH to 6.8.
After autoclaving, add 1.0 ml of vitamin B12 solution (2 mg/ml), 0.1% (final) of NaHCO3 and 2.0 mM (final) of Na2S2O3.
Micronutrient solution (SL7):
HCl (25%, v/v)  1.0     ml
ZnCl2  70.0     mg
MnCl2·4H2O  100.0     mg
H3BO3  60.0     mg
CoCl2·6H2O  200.0     mg
CuCl2·2H2O  20.0     mg
NiCl2·6H2O  20.0     mg
Na2MoO4·2H2O  40.0     mg
Distilled water  1.0     L