Medium data

Search for medium no. = [568].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

568 ROSEOSPIRA MEDIUM

KH₂PO₄	`0.5`	g
MgSO₄·7H₂O	`1.5`	g
NaCl	`20.0`	g
NH₄Cl	`0.6`	g
CaCl₂·2H₂O	`0.15`	g
Sodium pyruvate	`3.0`	g
Yeast extract	`0.4`	g
Ferric citrate (0.1%, w/v)	`5.0`	ml
Micronutrient solution (SL7) (see below)	`1.0`	ml
Distilled water	`1.0`	L

Adjust pH to 6.8.
After autoclaving, add 1.0 ml of vitamin B₁₂ solution (2 mg/ml), 0.1% (final) of NaHCO₃ and 2.0 mM (final) of Na₂S₂O₃.
Micronutrient solution (SL7):

HCl (25%, v/v)	`1.0`	ml
ZnCl₂	`70.0`	mg
MnCl₂·4H₂O	`100.0`	mg
H₃BO₃	`60.0`	mg
CoCl₂·6H₂O	`200.0`	mg
CuCl₂·2H₂O	`20.0`	mg
NiCl₂·6H₂O	`20.0`	mg
Na₂MoO₄·2H₂O	`40.0`	mg
Distilled water	`1.0`	L