Medium data

Search for medium no. = [561].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

561   THIORHODOCOCCUS BHEEMLICUM MEDIUM

KH2PO4  0.5     g
CaCl2·2H2O  0.15    g
MgSO4·7H2O  2.0     g
NH4Cl  0.34    g
NaCl  20.0     g
Yeast extract  0.4     g
Sodium pyruvate  3.0     g
Micronutrient solution SL7 (see Medium No. 533)  1.0     ml
Ferric citrate (0.1%, w/v)  5.0     ml
NaHCO3 (10%, w/v)  15.0     ml
Na2S·9H2O  1.0     mM
Na2S2O3·5H2O  6.0     mM
Distilled water  1.0     L
Adjust pH to 7.0.
Autoclave except NaHCO3, Na2S·9H2O and Na2S2O3·5H2O. After cooling, aseptically add filter-sterilized NaHCO3, Na2S·9H2O and Na2S2O3·5H2O solutions to the medium.