Medium data
Search for medium no. = [561].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
561 THIORHODOCOCCUS BHEEMLICUM MEDIUM
| KH2PO4 | 0.5 | g |
| CaCl2·2H2O | 0.15 | g |
| MgSO4·7H2O | 2.0 | g |
| NH4Cl | 0.34 | g |
| NaCl | 20.0 | g |
| Yeast extract | 0.4 | g |
| Sodium pyruvate | 3.0 | g |
| Micronutrient solution SL7 (see Medium No. 533) | 1.0 | ml |
| Ferric citrate (0.1%, w/v) | 5.0 | ml |
| NaHCO3 (10%, w/v) | 15.0 | ml |
| Na2S·9H2O | 1.0 | mM |
| Na2S2O3·5H2O | 6.0 | mM |
| Distilled water | 1.0 | L |
Adjust pH to 7.0.
Autoclave except NaHCO3, Na2S·9H2O and Na2S2O3·5H2O. After cooling, aseptically add filter-sterilized NaHCO3, Na2S·9H2O and Na2S2O3·5H2O solutions to the medium.
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