Medium data
Search for medium no. = [559].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
559 1/2 SME MEDIUM
| NaCl | 13.85 | g |
| MgSO4·7H2O | 3.5 | g |
| MgCl2·6H2O | 2.75 | g |
| KH2PO4 | 0.5 | g |
| (NH4)2SO4 | 0.25 | g |
| KCl | 0.33 | g |
| CaCl2·2H2O | 0.38 | g |
| NaBr | 0.05 | g |
| H3BO3 | 0.015 | g |
| SrCl2·6H2O | 7.5 | mg |
| KI | 0.05 | mg |
| NaHCO3 | 0.16 | g |
| Sulfur (powder) | 10.0 | g |
| Resazurin | 1.0 | mg |
| Na2S·9H2O | 0.5 | g |
| Distilled water | 1.0 | L |
Mix ingredients except Na2S·9H2O and distribute the medium into culture vessels under a stream of a N2-CO2 (4:1, v/v) gas mixture. Add Na2S·9H2O as a 5% solution, adjust pH to 6.0 and seal with butyl rubber stoppers. Autoclave at 110C for 1 hr. Prior to cultivation, replace the gas phase with a H2-CO2 (4:1, v/v) gas mixture. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
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