Medium data

Search for medium no. = [559].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

559   1/2 SME MEDIUM

NaCl  13.85    g
MgSO4·7H2O  3.5     g
MgCl2·6H2O  2.75    g
KH2PO4  0.5     g
(NH4)2SO4  0.25    g
KCl  0.33    g
CaCl2·2H2O  0.38    g
NaBr  0.05    g
H3BO3  0.015   g
SrCl2·6H2O  7.5     mg
KI  0.05    mg
NaHCO3  0.16    g
Sulfur (powder)  10.0     g
Resazurin  1.0     mg
Na2S·9H2O  0.5     g
Distilled water  1.0     L
Mix ingredients except Na2S·9H2O and distribute the medium into culture vessels under a stream of a N2-CO2 (4:1, v/v) gas mixture. Add Na2S·9H2O as a 5% solution, adjust pH to 6.0 and seal with butyl rubber stoppers. Autoclave at 110C for 1 hr. Prior to cultivation, replace the gas phase with a H2-CO2 (4:1, v/v) gas mixture. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).