Medium data

Search for medium no. = [558].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

558 MAGNETOSPIRILLUM MEDIUM (A)

Na₂HPO₄	`1.6`	g
KH₂PO₄	`1.0`	g
NH₄Cl	`0.5`	g
K₂SO₄	`0.06`	g
KNO₃	`0.51`	g
Trace x 10 solution (see below)	`0.1`	ml
Se/W solution (see below)	`1.0`	ml
Mg/Ca solution (see below)	`5.0`	ml
Na benzoate solution (see below)	`2.0`	ml
7 Vitamins solution (see below)	`0.5`	ml
FeSO₄ solution (see below)	`3.0`	ml
Distilled water	`1.0`	L

Adjust pH to 7.2 - 7.4.
Separately autoclave Trace x 10 solution, Se/W solution and Mg/Ca solution, and filter-sterilize Na benzoate solution, 7 vitamins solution and FeSO₄ solution. Aseptically add all solutions to the medium.
Trace x 10 solution:

H₃BO₃	`6.0`	mg
CuCl₂·2H₂O	`2.0`	mg
Distilled water	`100.0`	ml

Se/W solution:

NaOH	`0.05`	g
Na₂SeO₃	`0.2`	mg
Na₂WO₄·2H₂O	`0.4`	mg
Distilled water	`100.0`	ml

Mg/Ca solution:

MgCl₂·6H₂O	`2.0`	g
CaCl₂·2H₂O	`0.5`	g
Distilled water	`100.0`	ml

Na benzoate solution:

Sodium benzoate	`7.2`	g
Distilled water	`100.0`	ml

7 Vitamins solution:

Vitamin B₁₂	`4.0`	mg
D-Biotin	`2.0`	mg
D-Calcium pantothenate	`5.0`	mg
Thiamine·HCl	`10.0`	mg
p-Aminobenzoic acid	`10.0`	mg
Nicotinic acid	`20.0`	mg
Pyridoxine·HCl	`50.0`	mg
Distilled water	`100.0`	ml

FeSO₄ solution:

EDTA·2Na	`0.5`	g
FeSO₄·7H₂O	`0.05`	g
Distilled water	`100.0`	ml