Medium data

Search for medium no. = [558].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

558   MAGNETOSPIRILLUM MEDIUM (A)

Na2HPO4  1.6     g
KH2PO4  1.0     g
NH4Cl  0.5     g
K2SO4  0.06    g
KNO3  0.51    g
Trace x 10 solution (see below)  0.1     ml
Se/W solution (see below)  1.0     ml
Mg/Ca solution (see below)  5.0     ml
Na benzoate solution (see below)  2.0     ml
7 Vitamins solution (see below)  0.5     ml
FeSO4 solution (see below)  3.0     ml
Distilled water  1.0     L
Adjust pH to 7.2 - 7.4.
Separately autoclave Trace x 10 solution, Se/W solution and Mg/Ca solution, and filter-sterilize Na benzoate solution, 7 vitamins solution and FeSO4 solution. Aseptically add all solutions to the medium.
Trace x 10 solution:
H3BO3  6.0     mg
CuCl2·2H2O  2.0     mg
Distilled water  100.0     ml
Se/W solution:
NaOH  0.05    g
Na2SeO3  0.2     mg
Na2WO4·2H2O  0.4     mg
Distilled water  100.0     ml
Mg/Ca solution:
MgCl2·6H2O  2.0     g
CaCl2·2H2O  0.5     g
Distilled water  100.0     ml
Na benzoate solution:
Sodium benzoate  7.2     g
Distilled water  100.0     ml
7 Vitamins solution:
Vitamin B12  4.0     mg
D-Biotin  2.0     mg
D-Calcium pantothenate  5.0     mg
Thiamine·HCl  10.0     mg
p-Aminobenzoic acid  10.0     mg
Nicotinic acid  20.0     mg
Pyridoxine·HCl  50.0     mg
Distilled water  100.0     ml
FeSO4 solution:
EDTA·2Na  0.5     g
FeSO4·7H2O  0.05    g
Distilled water  100.0     ml