Medium data
Search for medium no. = [557].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
557 ALKALINE LB AGAR
| Tryptone (BD-Difco) | 10.0 | g |
| Yeast extract (BD-Difco) | 5.0 | g |
| NaCl | 15.0 | g |
| Na2CO3 | 5.0 | g |
| Agar | 15.0 | g |
| Distilled water | 1.0 | L |
Separately sterilize Na2CO3 by filtration and aseptically add to the medium. Check final pH to be about 8.5.
Copyright © 2024 Microbe Division (JCM) - All Rights Reserved