Medium data

Search for medium no. = [557].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

557   ALKALINE LB AGAR

Tryptone (BD-Difco)  10.0     g
Yeast extract (BD-Difco)  5.0     g
NaCl  15.0     g
Na2CO3  5.0     g
Agar  15.0     g
Distilled water  1.0     L
Separately sterilize Na2CO3 by filtration and aseptically add to the medium. Check final pH to be about 8.5.