Medium data

Search for medium no. = [548].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

548   GEOSPOROBACTER SUBTERRENEUS MEDIUM

Solution A:
Sea salts (Sigma)  10.0     g
NH4Cl  1.0     g
Resazurin  1.0     mg
Distilled water  920.0     ml
Mix ingredients and adjust pH to 7.2. Distribute 0.92 volume of the medium into tubes (e.g., 4.6 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave.
To complete the medium, add the following stock solutions [Filter-sterilize NaHCO3 solution and store under a N2-CO2 (4:1, v/v) gas mixture. The other solutions are autoclaved and stored anaerobically under a N2 atmosphere] to the medium (per 4.6 ml):
10% Yeast extract solution  0.05    ml
1 M Glucose  0.1     ml
5% NaHCO3 solution  0.2     ml
2% Na2S·9H2O solution  0.05    ml