Medium data

Search for medium no. = [546].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

546   HALOPHILE YEAST EXTRACT MEDIUM

NaCl  195.0     g
MgCl2·6H2O  32.5     g
MgSO4·7H2O  50.8     g
CaCl2·2H2O  0.8     g
KCl  5.0     g
NaHCO3  0.16    g
NaBr  0.6     g
Yeast extract  5.0     g
Add components except NaHCO3 to distilled water and bring volume to 1.0 L. Adjust pH to 8.0 with 1 M NaOH and autoclave. Filter-sterilize 8% (w/v) NaHCO3 and add to the medium aseptically.