Medium data
Search for medium no. = [546].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
546 HALOPHILE YEAST EXTRACT MEDIUM
| NaCl | 195.0 | g |
| MgCl2·6H2O | 32.5 | g |
| MgSO4·7H2O | 50.8 | g |
| CaCl2·2H2O | 0.8 | g |
| KCl | 5.0 | g |
| NaHCO3 | 0.16 | g |
| NaBr | 0.6 | g |
| Yeast extract | 5.0 | g |
Add components except NaHCO3 to distilled water and bring volume to 1.0 L. Adjust pH to 8.0 with 1 M NaOH and autoclave. Filter-sterilize 8% (w/v) NaHCO3 and add to the medium aseptically.
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