Medium data

Search for medium no. = [542].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

542 MODIFIED BROCK'S BASAL SALTS-YEAST EXTRACT MEDIUM

(NH₄)₂SO₄	`1.3`	g
KH₂PO₄	`0.28`	g
MgSO₄·7H₂O	`0.25`	g
CaCl₂·2H₂O	`0.07`	g
FeCl₃·6H₂O	`2.0`	mg
MnCl₂·4H₂O	`1.8`	mg
Na₂B₄O₇·10H₂O	`4.5`	mg
ZnSO₄·7H₂O	`0.22`	mg
CuCl₂·2H₂O	`0.05`	mg
Na₂MoO₄·2H₂O	`0.03`	mg
VOSO₄·xH₂O	`0.03`	mg
CoSO₄·7H₂O	`0.01`	mg
Yeast extract (BD-Difco)	`5.0`	g
Distilled water	`1.0`	L

Adjust pH to 9.0.
Autoclave and remove precipitate, if necessary.

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