Medium data
Search for medium no. = [525].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
525 ACIDIANUS SP. JP7 MEDIUM
| (NH4)2SO4 | 1.5 | g |
| KH2PO4 | 0.25 | g |
| MgSO4·7H2O | 0.25 | g |
| FeCl3·6H2O | 0.02 | g |
| MnCl2·4H2O | 1.8 | mg |
| Na2B4O7·10H2O | 4.5 | mg |
| ZnSO4·7H2O | 0.22 | mg |
| CuCl2·2H2O | 0.05 | mg |
| Na2MoO4·2H2O | 0.03 | mg |
| VOSO4·xH2O | 0.03 | mg |
| CoSO4·7H2O | 0.01 | mg |
| Yeast extract (BD-Difco) | 0.1 | g |
| Chalcopyrite | 10.0 | g |
Dissolve ingredients except chalcopyrite in 500 ml distilled water, adjust pH to 0.8 with 10 N H2SO4 and autoclave. Pulp chalcopyrite in 500 ml distilled water and steam for 1 hr on each of 3 successive days. Combine the medium and the chalcopyrite concentrate.
Comment: Chalcopyrite can be replaced with arsenopyrite or pyrite.
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