Medium data
Search for medium no. = [514].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
514 GLUCOSE SULFIDE MEDIUM
| Glucose | 0.15 | g |
| Yeast extract | 1.0 | g |
| (NH4)2SO4 | 0.5 | g |
| CaCO3 | 0.1 | g |
| Ca(NO3)2 | 0.1 | g |
| KCl | 0.05 | g |
| K2HPO4 | 0.05 | g |
| MgSO4·7H2O | 0.05 | g |
| Na2S·9H2O | 0.2 | g |
| Vitamin solution (see below) | 10.0 | ml |
| Agar | 15.0 | g |
| Distilled water | 990.0 | ml |
Add components except Vitamin solution to distilled water and bring volume to 990 ml. Adjust pH to 7.3. After autoclaving, aseptically add filter-sterilized Vitamin solution.
Vitamin solution:
| Biotin | 2.0 | mg |
| Folic acid | 2.0 | mg |
| Pyridoxine·HCl | 10.0 | mg |
| Thiamine·HCl·2H2O | 5.0 | mg |
| Riboflavin | 5.0 | mg |
| Nicotinic acid | 5.0 | mg |
| Calcium pantothenate | 5.0 | mg |
| p-Aminobenzoic acid | 1.0 | mg |
| myo-Inositol | 1.0 | mg |
| Vitamin B12 | 0.01 | mg |
| Distilled water | 1.0 | L |
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