Medium data
Search for medium no. = [510].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
510 NMS MEDIUM
| MgSO4·7H2O | 1.0 | g |
| CaCl2·2H2O | 0.13 | g |
| Ferric citrate | 4.0 | mg |
| KNO3 | 1.0 | g |
| Trace element solution (see below) | 0.5 | ml |
| 20 x Phosphate solution (see below) | 50.0 | ml |
| Distilled water | 1.0 | L |
Adjust pH to 6.8. Separately autoclave 20 x Phosphate solution and add aseptically to the medium. Cultivate under a methane-air (50:50) atmosphere.
Trace element solution:
| EDTA·2Na | 500.0 | mg |
| FeSO4·7H2O | 200.0 | mg |
| ZnSO4·7H2O | 10.0 | mg |
| MnCl2·4H2O | 3.0 | mg |
| H3BO3 | 30.0 | mg |
| CoCl2·6H2O | 20.0 | mg |
| CuCl2·2H2O | 1.0 | mg |
| NiCl2·6H2O | 2.0 | mg |
| Na2MoO4·2H2O | 3.0 | mg |
| Distilled water | 1.0 | L |
20 x Phosphate solution:
| KH2PO4 | 5.44 | g |
| Na2HPO4·12H2O | 14.3 | g |
| Distilled water | 1.0 | L |
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