Medium data

Search for medium no. = [507].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

507 METHANOCELLA MEDIUM

Use Solution A of Medium No. 284 supplemented with 0.1 g sodium acetate and bring final volume to 1.0 L. Replace the gas phase with H₂-CO₂ (80:20, v/v) and pressurize an inoculated bottle to 150 kPa with the same gas mixture.
Comment: For cultivation of strain JCM 13418, incubate with a 10% inoculum.