Medium data

Search for medium no. = [506].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


(NH4)2SO4  1.3     g
KH2PO4  0.28    g
MgSO4·7H2O  0.28    g
CaCl2·2H2O  0.074   g
Yeast extract  0.1     g
Trypticase peptone (BD-BBL)  2.0     g
Soluble starch  2.0     g
L-Cystine  0.3     g
Trace elements (see below)  1.0     ml
FeCl3 solution (see below)  1.0     ml
Resazurin  1.0     mg
Distilled water  1.0     L
Mix components thoroughly, adjust pH to 6.5 and autoclave under a N2 atmosphere. After cooling, add per liter the following solution (autoclaved and stored under a N2 atmosphere):
5% Na2S·9H2O solution (adjusted pH to 7.0)  6.0     ml
Trace elements:
MnSO4·xH2O  2.2     mg
ZnSO4·7H2O  0.5     mg
H3BO3  0.5     mg
CuSO4·5H2O  0.016   mg
Na2MoO4·2H2O  0.025   mg
CoSO4·7H2O  0.046   mg
Distilled water  1.0     L
Dissolve at 1,000-fold concentration in acidified water (0.5 ml concentrated H2SO4 per liter), then dilute.
FeCl3 solution:
FeCl3·6H2O  0.28    g
Distilled water  1.0     L

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