Medium data

Search for medium no. = [500].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

500   TYE MEDIUM

(NH4)2SO4  1.3     g
KH2PO4  0.28    g
MgSO4·7H2O  0.25    g
CaCl2·2H2O  0.07    g
MnCl2·4H2O  1.8     mg
Na2B4O7·10H2O  4.5     mg
ZnSO4·7H2O  0.22    mg
CuSO4·5H2O  0.05    mg
Na2MoO4·2H2O  0.03    mg
CoCl2·6H2O  0.01    mg
Yeast extract  2.0     g
Trypticase peptone (BD-BBL)  4.0     g
Na2S·9H2O  0.5     g
Distilled water  1.0     L
Mix ingredients except Na2S·9H2O and adjust pH to 7.0 with 1 N NaOH. Distribute the medium into culture vessels under a N2 gas atmosphere, add Na2S·9H2O (as a 5% solution), seal with butyl rubber stoppers and autoclave.