Medium data
Search for medium no. = [500].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
500 TYE MEDIUM
| (NH4)2SO4 | 1.3 | g |
| KH2PO4 | 0.28 | g |
| MgSO4·7H2O | 0.25 | g |
| CaCl2·2H2O | 0.07 | g |
| MnCl2·4H2O | 1.8 | mg |
| Na2B4O7·10H2O | 4.5 | mg |
| ZnSO4·7H2O | 0.22 | mg |
| CuSO4·5H2O | 0.05 | mg |
| Na2MoO4·2H2O | 0.03 | mg |
| CoCl2·6H2O | 0.01 | mg |
| Yeast extract | 2.0 | g |
| Trypticase peptone (BD-BBL) | 4.0 | g |
| Na2S·9H2O | 0.5 | g |
| Distilled water | 1.0 | L |
Mix ingredients except Na2S·9H2O and adjust pH to 7.0 with 1 N NaOH. Distribute the medium into culture vessels under a N2 gas atmosphere, add Na2S·9H2O (as a 5% solution), seal with butyl rubber stoppers and autoclave.
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