Medium data

Search for medium no. = [489].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


Casitone (BD-Difco)  20.0     g
Yeast extract  5.0     g
Na2HPO4  5.0     g
MgCl2·6H2O  1.10    g
Urea  1.0     g
Sodium thioglycolate  0.75    g
Resazurin  0.5     mg
Distilled water  1.0     L
Mix components except urea and sodium thioglycolate, bring to a boil for a few seconds and cool to room temperature under a N2-CO2 (4:1, v/v) gas mixture. Add sodium thioglycolate, dispense the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add 10% urea solution (filter-sterilized and stored under a N2 gas atmosphere) to the medium and adjust pH to 7.4.