Medium data
Search for medium no. = [489].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
489 CATONELLA UREILYTICA MEDIUM
| Casitone (BD-Difco) | 20.0 | g |
| Yeast extract | 5.0 | g |
| Na2HPO4 | 5.0 | g |
| MgCl2·6H2O | 1.10 | g |
| Urea | 1.0 | g |
| Sodium thioglycolate | 0.75 | g |
| Resazurin | 0.5 | mg |
| Distilled water | 1.0 | L |
Mix components except urea and sodium thioglycolate, bring to a boil for a few seconds and cool to room temperature under a N2-CO2 (4:1, v/v) gas mixture. Add sodium thioglycolate, dispense the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add 10% urea solution (filter-sterilized and stored under a N2 gas atmosphere) to the medium and adjust pH to 7.4.
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