Medium data

Search for medium no. = [479].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

479   THERMODESULFOVIBRIO MEDIUM

Solution A:
Use Solution A of Medium No. 284 without yeast extract. Prepare the culture vessels containing the solution as described in the recipe of Medium No. 284.
Solution B:
Sodium lactate  2.2     g
Distilled water  50.0     ml
Autoclave and store under a N2 atmosphere.
Solution C:
Na2SO4  2.8     g
Distilled water  50.0     ml
Autoclave and store under a N2 atmosphere.
To complete the medium, add 0.05 volume of Solutions B and C to the culture vessels containing Solution A. Prior to inoculation, aseptically and anaerobically add 0.01 volume of L-cysteine·HCl·H2O and Na2S·9H2O solutions to the medium (3% solutions, autoclaved and stored under a N2 atmosphere).
Comment: For strains JCM 13213, JCM 13214, JCM 13215 and JCM 13216, incubate with a 10% inoculum.