Medium data

Search for medium no. = [460].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

460 FLUID THIOGLYCOLLATE MEDIUM

Trypticase peptone (BD-BBL)	`15.0`	g
L-Cystine	`0.5`	g
Glucose	`2.0`	g
Yeast extract (BD-Difco)	`5.0`	g
NaCl	`2.5`	g
Sodium thioglycollate	`0.5`	g
Resazurin	`0.001`	g
Bacto agar (BD-Difco) (for solid medium)	`15.0`	g
Distilled water	`1.0`	L

Adjust pH to 7.1 with Na₂CO₃ (10%, w/v), if necessary. Prepare medium under a N₂ atmosphere.