Medium data
Search for medium no. = [457].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
457 ARTIFICIAL SEAWATER FOR HALOPHILES
| NaCl | 195.0 | g |
| MgCl2·6H2O | 35.0 | g |
| MgSO4·7H2O | 50.0 | g |
| KCl | 5.0 | g |
| NaHCO3 | 0.25 | g |
| NaNO3 | 1.0 | g |
| CaCl2·2H2O | 0.5 | g |
| KH2PO4 | 0.05 | g |
| NH4Cl | 0.03 | g |
| Yeast extract | 0.05 | g |
| Sodium pyruvate | 1.0 | g |
Add components except NaHCO3 and sodium pyruvate to distilled water and bring volume to 1.0 L. Adjust pH to 7.4 with 1 M Tris base and autoclave. Filter-sterilize 8% (w/v) NaHCO3 and 25% (w/v) sodium pyruvate solutions and add to the medium aseptically.
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