Medium data

Search for medium no. = [457].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

457   ARTIFICIAL SEAWATER FOR HALOPHILES

NaCl  195.0     g
MgCl2·6H2O  35.0     g
MgSO4·7H2O  50.0     g
KCl  5.0     g
NaHCO3  0.25    g
NaNO3  1.0     g
CaCl2·2H2O  0.5     g
KH2PO4  0.05    g
NH4Cl  0.03    g
Yeast extract  0.05    g
Sodium pyruvate  1.0     g
Add components except NaHCO3 and sodium pyruvate to distilled water and bring volume to 1.0 L. Adjust pH to 7.4 with 1 M Tris base and autoclave. Filter-sterilize 8% (w/v) NaHCO3 and 25% (w/v) sodium pyruvate solutions and add to the medium aseptically.