Medium data

Search for medium no. = [455].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

455   ALKALI-REINFORCED CLOSTRIDIAL AGAR

Reinforced clostridial agar (Sigma)  51.0     g
Distilled water  900.0     ml
After autoclaving, aseptically add 100 ml of a 10% Na2CO3 solution to the medium. Check final pH to be about 10.0.