Medium data

Search for medium no. = [446].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

446   MJTSO MEDIUM

NaCl  3.0     g
MgCl2·6H2O  0.34    g
MgSO4·7H2O  0.42    g
KCl  0.05    g
K2HPO4  0.14    g
NH4Cl  0.25    g
CaCl2·2H2O  0.07    g
Trace mineral solution (see below)  10.0     ml
Na2S2O3·5H2O  1.0     g
Yeast extract  0.1     g
Sulfur (powder)  3.0     g
NaHCO3  1.0     g
Distilled water  1.0     L
Mix ingredients except NaHCO3 and sulfur, adjust pH to 7.5 with NaOH and autoclave. Filter-sterilize 8% NaHCO3 solution and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 20 ml of medium in 120 ml serum bottles containing 0.06 g sulfur) under a stream of CO2-O2 (93:7, v/v) gas mixture, seal with butyl rubber stoppers and readjust pH to 7.5 with NaOH. Pressurize the inoculated vessel to 100 kPa CO2-O2 (93:7, v/v).
Trace mineral solution:
EDTA·2Na  0.5     g
CoCl2·6H2O  0.15    g
MnCl2·4H2O  0.1     g
FeSO4·7H2O  0.1     g
ZnCl2  0.1     g
AlCl3·6H2O  0.04    g
Na2WO2·2H2O  0.03    g
CuCl2·2H2O  0.02    g
NiSO4·6H2O  0.02    g
H2SeO3  0.01    g
H3BO3  0.01    g
Na2MoO4·2H2O  0.01    g
Distilled water  1.0     L



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