Medium data
Search for medium no. = [423].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
423 PYG MEDIUM (B)
| Polypepton (Nihon Pharm. Co.) | 5.0 | g |
| Tryptone (BD-Difco) | 5.0 | g |
| Yeast extract (BD-Difco) | 10.0 | g |
| Glucose | 10.0 | g |
| Salts solution (see below) | 40.0 | ml |
| Distilled water | 960.0 | ml |
Adjust pH to 7.2.
Salts solution:
| CaCl2 | 0.2 | g |
| MgSO4·7H2O | 0.4 | g |
| K2HPO4 | 1.0 | g |
| KH2PO4 | 1.0 | g |
| NaHCO3 | 10.0 | g |
| NaCl | 2.0 | g |
| Distilled water | 1.0 | L |
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