Medium data

Search for medium no. = [423].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

423   PYG MEDIUM (B)

Polypepton (Nihon Pharm. Co.)  5.0     g
Tryptone (BD-Difco)  5.0     g
Yeast extract (BD-Difco)  10.0     g
Glucose  10.0     g
Salts solution (see below)  40.0     ml
Distilled water  960.0     ml
Adjust pH to 7.2.
Salts solution:
CaCl2  0.2     g
MgSO4·7H2O  0.4     g
K2HPO4  1.0     g
KH2PO4  1.0     g
NaHCO3  10.0     g
NaCl  2.0     g
Distilled water  1.0     L