Medium data
Search for medium no. = [414].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
414 MODIFIED SOB MEDIUM
| KH2PO4 | 1.0 | g |
| K2HPO4 | 1.0 | g |
| NH4Cl | 1.0 | g |
| CaCl2·2H2O | 0.1 | g |
| MgCl2·6H2O | 0.2 | g |
| Na2S2O3·5H2O | 1.55 | g |
| Trace metal solution (see below) | 1.0 | ml |
| NaHCO3 | 2.0 | g |
Dissolve KH2PO4, K2HPO4, NH4Cl and Na2S2O3·5H2O in 500 ml distilled water. Separately dissolve CaCl2·2H2O, MgCl2·6H2O and Trace metal solution in 460 ml distilled water. Autoclave both solutions, mix them after cooling, and add 40 ml filter-sterilized 5% NaHCO3 solution to the medium. Adjust pH to 7.0 with HCl, if necessary.
Trace metal solution:
| EDTA·2Na | 50.0 | g |
| ZnSO4·7H2O | 2.2 | g |
| CaCl2·2H2O | 7.3 | g |
| MgCl2·6H2O | 2.5 | g |
| CoCl2·6H2O | 0.5 | g |
| (NH4)6Mo7O24·4H2O | 0.5 | g |
| FeSO4·7H2O | 5.0 | g |
| CuSO4·5H2O | 0.2 | g |
| NaOH | 11.0 | g |
| Distilled water | 1.0 | L |
Adjust pH to 6.0.
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