Medium data
Search for medium no. = [402].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
402 HALOCOCCUS DOMBOWSKII MEDIUM
| Casamino acids (BD-Difco) | 5.0 | g |
| Yeast extract | 5.0 | g |
| Tris base | 12.1 | g |
| KCl | 2.0 | g |
| MgCl2·6H2O | 20.0 | g |
| CaCl2·2H2O | 0.2 | g |
| NaCl | 200.0 | g |
Add components to distilled water and bring volume to 1.0 L.
Adjust pH to 7.4.
For preparation of solid medium, add 20.0 g/L agar.
Comment: For cultivation of JCM 19729, supplement with 10.0 g/L sea salts (Sigma).
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