Medium data

Search for medium no. = [402].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

402   HALOCOCCUS DOMBOWSKII MEDIUM

Casamino acids (BD-Difco)  5.0     g
Yeast extract  5.0     g
Tris base  12.1     g
KCl  2.0     g
MgCl2·6H2O  20.0     g
CaCl2·2H2O  0.2     g
NaCl  200.0     g
Add components to distilled water and bring volume to 1.0 L.
Adjust pH to 7.4.
For preparation of solid medium, add 20.0 g/L agar.
Comment: For cultivation of JCM 19729, supplement with 10.0 g/L sea salts (Sigma).